endonuclease是什么意思

2023-12-26 05:56:17

endonuclease

美[ˌendoʊnjuːkliːˌeɪs] 英[endənjuːklɪeɪs]

n. [生化]核酸内切酶

Noun

a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes

A ,sad sequence ;B ,restriction endonuclease cutting sites of sad. 段,与序列分析结果相一致(图略)。
Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid. 方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
Mimics of both types of BCR ABL cDNA were achieved and the validity was verified with restriction endonuclease. 经酶切分析证明 ,两型BCR ABLmRNA均可通过此方法得到相应的cDNA参照物。
Results The result of restriction endonuclease digestion was accordance with the anticipated objective strap size . 结果酶切结果与预期目的条带大小相符；
PCR amplification and restriction endonuclease digestion was used to identify deleted DNA fragments. 应用PCR技术与限制性内切酶酶切相结合的方法鉴定缺失子。
Method The two clones of OSCP gene were modified by restriction endonuclease and recombined by T_4DNA ligase. 方法通过限制性核酸内切酶将发生有义突变的两个OSCP基因的DNA克隆进行改造,并进行基因重组。

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